Steric-exclusion chromatography of purified AKAP12. His-tagged AKAP12 was purified and then resolved by steric-exclusion chromatography performed on an AKTA FPLC fitted with HiPrep Sephacryl-S400 (16/60) column. The presence of supermolecular oligomers of AKAP12 was established by SDS-PAGE and immunoblotting of samples from the chromatography. The resolved, transferred proteins were stained with anti-AKAP12 antibodies. Marker protein mobilities were employed to establish the Mr of AKAP12 by elution position (inset). The results displayed are representative of three separate experiments performed on as many separate cell cultures.
Gao et al. Journal of Molecular Signaling 2011 6:3 doi:10.1186/1750-2187-6-3