Table 1 |
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Primers used for PCR |
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Transcript (Primer Name) |
Forward Primer |
Reverse Primer |
Product Size |
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H. E4BP4 (HUMANE4BP4-742 & 991) |
5'ATGGGGAATTCTTTCTCTGG3' |
5'CTTTGATCCGGAGCTTGTGT3' |
250 bp |
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M. E4BP4 (Mouse E4BP4-742 &1117) |
5'ATGGGAAGCTCTTTCTCCACT3' |
5'TACCCGAGGTTCCATGTTTC3' |
376 bp |
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Bim (BIM 5/6 SENSE & ANTI) |
5'CAGATATGCGCCCAGAGATA3' |
5'ACCAGGCGGACAATGTAAC3' |
163 bp |
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Puma (PUMA SENSE & ANTI) |
5'AAGAGCAAATGAGCCAAACG3' |
5'GCAGAGCACAGGATTCACAG3' |
181 bp |
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β-actin (B-ACTIN-130 SENSE & ANTI) |
5'AGTCCTCTCCCAAGTCCACA3' |
5'CACGAAGGCTCATCATTCAA3' |
130 bp |
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Beach et al. Journal of Molecular Signaling 2011 6:13 doi:10.1186/1750-2187-6-13 |
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