Endogenous Wnt signaling component expression during myogenic differentiation of C2C12 cells. Wnt signaling molecule expression was measured by quantitative RT-PCR. After 2 or 4 days culture in differentiation medium, cells were harvested, total RNA prepared and relative gene expression quantified by real-time PCR as follows: (A) wingless-related MMTV integration, Wnt; secreted frizzled-related protein, Sfrp; and porcupine, Porcn. (B) frizzled, Fzd; and low density lipoprotein receptor-related protein, Lrp. (C) cyclin D, Ccnd; myelocytomatosis oncogene, Myc; fos-like antigen 1, Fosl1; casein kinase 1, alpha 1, Csnk1a1; catenin, beta 1, Ctnnb1; dishevelled 1, Dvl1; glycogen synthase kinase 3 beta, Gsk3b; transcription factor 3, Tcf3; and transducin-like enhancer of split 2, Tle2. Day 2 and 4 time points were normalized to expression levels at day 0. Data are from three independent experiments, *P < 0.005 and † P < 0.05.
Tanaka et al. Journal of Molecular Signaling 2011 6:12 doi:10.1186/1750-2187-6-12