Research article
Canonical Wnt signaling is involved in switching from cell proliferation to myogenic differentiation of mouse myoblast cells
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* Corresponding author: Tsutomu Nohno nohno@bcc.kawasaki-m.ac.jp
Department of Molecular and Developmental Biology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan
Journal of Molecular Signaling 2011, 6:12 doi:10.1186/1750-2187-6-12
Published: 5 October 2011Additional files
Additional file 1:
Real-time PCR analysis. (A) Array layout of 96 well RT-PCR kit. (B) Heat map of PCR results between day 2 and 0 samples in differentiation medium. (C) Heat map of PCR results between day 4 and 0 samples in differentiation medium. Note: the scales are not identical in panels B and C.
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Additional file 2:
Real-time PCR results (Day 2 vs control). Array results of real-time PCR analysis. n = 3 for the Day 2 group and n = 4 for the control group.
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Additional file 3:
Real-time PCR results (Day 4 vs control). Array results of real-time PCR analysis. n = 3 for the Day 4 group and n = 4 for the control group.
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Additional file 4:
Effect of Wnt expression on cell differentiation. C2C12 cells were transfected with Wnt cDNAs in pcDNA3.2 and cultured in proliferation medium for 2 days. Cells were fixed, troponin T immunostained and positive cell nuclei were counted. *P < 0.03 vs. control.
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Time-lapse movie (control). The movie consists of micrographs taken at 5 min intervals for 16 h. Cells were transfected with an eGFP-expressing plasmid.
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Time-lapse movie (Wnt4). The movie consists of micrographs taken at 5 min intervals for 16 h. Cells were transfected with a Wnt4-expressing plasmid.
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Time-lapse movie (Wnt5a). The movie consists of micrographs taken at 5 min intervals for 16 h. Cells were transfected with a Wnt5a-expressing plasmid.
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Time-lapse movie (Wnt3a). The movie consists of micrographs taken at 5 min intervals for 16 h. Cells were transfected with a Wnt3a-expressing plasmid.
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