Effect of G17 on GSK3βSer9 phosphorylation in gastric cancer cells. (A) Confluent AGSE cells were treated in the absence (-) or presence (+) of 100 nM G17 in serum free media for the indicated periods of time. Equal amounts of total protein were fractionated by SDS-PAGE and subjected to Western Blot analysis utilizing antibodies against phospho-GSK3βSer9, total GSK3β, phospho-AKTSer473 and total AKT. (B) AGSE cells were treated with increasing concentrations of G17 for 1 hour followed by Western Blot analysis with the antibodies indicated. (C) & (D) Western Blot analysis of AGSE cells with the indicated antibodies, treated with 100 nM G17 for 1 hour following an overnight pretreatment with 100 nM YM 022 (C) or 1 μM Wortmannin (D). (E) MKN45 cells treated as in A were harvested at different time points following G17 treatment and analyzed by Western Blots utilizing the antibodies indicated.
Mishra et al. Journal of Molecular Signaling 2010 5:9 doi:10.1186/1750-2187-5-9