Research article
A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1
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* Corresponding author: Arun K Rishi Rishia@Karmanos.org
1 Department of Internal Medicine, Wayne State University and John D. Dingell VA Medical Center, Room B4325, 4646 John R, Detroit, MI 48201, USA
2 Karmanos Cancer Institute, Wayne State University and John D. Dingell VA Medical Center, Room B4325, 4646 John R, Detroit, MI 48201, USA
3 Department of Surgery, Wayne State University, and John D. Dingell VA Medical Center, Room B4245, 4646 John R, Detroit, MI 48201, USA
4 Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02115, UK
5 Department of Biochemistry, University of Western Ontario Schulich School of Medicine and Dentistry, 4th Floor Victoria Research Labs, A4-130a 800 Commissioners Road East, London, ON N6C 2V5, UK
6 Department of Obstetrics & Gynecology, University of Western Ontario Schulich School of Medicine and Dentistry, 4th Floor Victoria Research Labs, A4-130a 800 Commissioners Road East, London, ON N6C 2V5, UK
Journal of Molecular Signaling 2010, 5:7 doi:10.1186/1750-2187-5-7
Published: 1 July 2010Additional files
Additional file 1:
Supplemental Figure. CARP-1 (651-759) binds with TAZ (1-120). Cells were transfected with plasmid encoding flag-tagged wild-type TAZ in combination vector or plasmids expressing noted myc-His-tagged CARP-1 mutant proteins (panel A), or the indicated combinations of myc-His-tagged CARP-1 (651-759) and gst-tagged TAZ mutants (panel B). The immunoprecipitation and western blotting were carried out using noted antibodies essentially as in figure 3. In panel A, the membranes were subsequently probed with anti-myc-tag antibodies to assess expression of respective CARP-1 mutant protein.
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