Specific knockdown of BRCA2 mRNA expression measured by RT-qPCR in MCF-7, MCF-10a and MDA-MB-231 breast cell lines after BRCA2-siRNA, BRCA2-siRNA + daidzein, and BRCA2-siRNA + genistein 72-h treatments in comparison to controls. The ΔΔCt method and normalization by comparison with controls were used. Values are means ± SD for n = 3 assays. Statistical analysis was carried out by Student t-test (*, P < 0.05; †, P < 0.01).
Satih et al. Journal of Molecular Signaling 2009 4:3 doi:10.1186/1750-2187-4-3