Figure 5.

Time-course of catharanthine and vindoline accumulation in cells irradiated with UV-B in C. roseus cell suspension cultures. Six-day-old suspension cultured cells of C. roseus were irradiated with UV-B light for 5 min or left untreated and harvested at indicated times. A, Catharanthine and B, vindoline accumulation was detected by HPLC as described in materials and methods. Values are represented as the means ± SD (n = 3).