Figure 3.

Effect of UV-B irradiation on growth of C. roseus suspension cultures. Actively growing cells from 4-day-old cultures were inoculated into fresh MS medium supplemented with growth regulators and cultured under 16 h day/8 h dark photoperiod. Cells were irradiated with UV-B on day 4 or day 6 after sub-culture or left untreated as described in materials and methods. Cell growth was measured every day by determining the fresh weight of the harvested cells. Values are expressed as the means ± SD (n = 3).