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Enhanced catharanthine and vindoline production in suspension cultures of Catharanthus roseus by ultraviolet-B light

Shilpa Ramani email and Chelliah Jayabaskaran email

Department of Biochemistry, Indian Institute of Science, Bangalore – 560012, India

author email corresponding author email

Journal of Molecular Signaling 2008, 3:9doi:10.1186/1750-2187-3-9

Published: 25 April 2008

Abstract

Suspension cultures of Catharanthus roseus were used to evaluate ultraviolet-B (UV-B) treatment as an abiotic elicitor of secondary metabolites. A dispersed cell suspension culture from C. roseus leaves in late exponential phase and stationary phase were irradiated with UV-B for 5 min. The stationary phase cultures were more responsive to UV-B irradiation than late exponential phase cultures. Catharanthine and vindoline increased 3-fold and 12-fold, respectively, on treatment with a 5-min UV-B irradiation.


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