Additional file 1.Determining specificity of SHP-1 antibody. (A) SHP-1 was immunoprecipitated from HUVEC lysates using an antibody and the "Catch and release column" from Upstate. It was immunostained with an HRP-conjugated secondary antibody to SHP-1. (B) SHP-1 was immunoprecipitated as above. It was immunostained with an antibody to SHP-2. Format: TIFF Size: 55KB Download file Bhattacharya et al. Journal of Molecular Signaling 2008 3:8 doi:10.1186/1750-2187-3-8 |