Figure 4.

Knockdown of SHP-1 using siRNA and its effect on DNA synthesis. (A) HUVEC were transfected with scrambled control or SHP-1 siRNA using oligofectamine and protein levels checked after 48 h. β actin served as a loading control. SHP-2 levels were not affected. NIH Image quantitation data normalized with respect to b actin is shown. (B) HUVEC were transfected with scrambled control or SHP-1 siRNA using oligofectamine. After 48 h, the cells were plated on 24 well plates and [³H]thymidine incorporation assay carried out as described in Materials and Methods. Data represent average of five independent determinations each in triplicate (P = 0.004).