Figure 3.

SHP-1 phosphatase assay. Phosphatase assay was performed using pNNP. Serum starved HUVEC was pretreated with PP2 or PP3, 5 uM for 1 h followed by treatment with VEGF 10 ng/ml for 5 min and immunoprecipitated with antibody against SHP-1 using, "catch and release column," from Upstate. One part was run on a gel and the other part was used for the phosphatase assay. Data represent average of three independent determinations normalized with respect to negative control (P = 0.001).