Figure 3.

Effect of Phenylephrine on Intracellular Calcium Levels in Human Aortic Smooth Muscle Cells. Human aortic smooth muscle cells were loaded with Calcium Green 1 AM for 1 hr. The ability of 25 uM phenylephrine to increase intracellular calcium was studied alone and following treatment with either 1 nM prazosin or 30 nM BMY 7378. Experiments were carried out as described in Methods. The results of a typical imaging study are presented along with a graphical summary of the statistical analysis of four independent experiments. Data were analyzed by a one-way ANOVA followed by post hoc testing. * Indicates a statistically significant difference from the untreated control.