Figure 1.

Overlay analysis of A kinase-anchoring proteins in A431 and HEK cells. Cell lysates (50 μg of protein/lane) of A431 or HEK cells were subjected to SDS-polyacrylamide gel electrophoresis, the resolved proteins transferred to nitrocellulose blots, and the blots subjected to renaturation and stained with A-kinase RII subunit for 2 h at room temperature. After washing, the presence of AKAPs was detected using an goat anti-RII antibody. The resultant blots also were stained with antibodies specific for AKAP12 or AKAP5.