Table 5

Expression of Wnt gene in mouse totipotent teratocarcinoma embryonic cell (F9), human embryonic stem cell (H7), and human mesenchymal cells (hM).
gene\cell
 F9
 H7
 hM
Wnt 1
 +
 +
 -
Wnt 2
 -
 +
 +
Wnt 2b
 +
 +
 +
Wnt 3
 +
 +
 +
Wnt 3a
 +
 +
 +
Wnt 4
 +
 +
 +
Wnt 5a
 +
 +
 +
Wnt 5b
 +
 +
 +
Wnt 6
 +
 +
 +
Wnt 7a
 +
 +
 +
Wnt 7b
 +
 +
 +
Wnt 8a
 +
 +
 -
Wnt 8b
 +
 +
 -
Wnt 9a
 +
 +
 +
Wnt 9b
 +
 +
 -
Wnt 10a
 +
 +
 -
Wnt 10b
 +
 +
 +
Wnt 11
 +
 +
 -
Wnt 16
 +
 +
 +

RNA was extracted from F9, H7 and hM and reverse-transcribed to obtain first strand cDNA. Specific primers were used in PCR to amplify each Wnt cDNA. Amplified products were purified and confirmed by DNA sequencing.

Okoye et al. Journal of Molecular Signaling 2008 3:16   doi:10.1186/1750-2187-3-16