Figure 5.

siRNA-mediated silencing of CK2β leads to higher viral protein content. (a) A549 cells were transfected with either control (white bars) or CK2β (black bars) siRNA. After 48 h, cells were inoculated at an MOI of 1 and incubated for 9 h or 24 h before Western blot analysis. Viral proteins were detected with a goat anti-IVA (H1N1) polyclonal antibody. Loading was controlled with a mAb against β-actin. Bands of viral protein were quantified as a percentage of control values normalized to the loading control. Shown are the mean ± SE from 3 independent experiments. (b) Cells were treated as above. The percentage of NP-expressing cells was measured at 24 h p.i. by flow cytometry (FACS) using an anti-NP mAb. The experiments were performed in duplicate.