Table 1

Production of Ig classes and IgG subclasses in WT mice or mice deficient in Gα12 and/or Gα13

Thymus-dependent Ig production
 Thymus-independent Ig production


WT
 Gα12 +/-
 Gα12 -/-
 Gα13 +/-
 Gα12/Gα13+/-
 WT
 Gα12 +/-
 Gα12 -/-
 Gα13 +/-
 Gα12/Gα13 +/-
IgG
 ++
 ++
 +
 +
 +
 ++++
 +++
 +
 ++
 +
IgG1
 +++
 +++
 +
 ++
 +
 +
 +
 +
 +
 +
IgG2a
 ++
 +
 +
 +
 +
 +
 +
 +
 +
 +
IgG2b
 ++
 ++
 +
 +
 +
 ++++
 +++
 +
 ++
 +
IgG3
 t
 t
 t
 t
 t
 +
 +
 +
 +
 ++
IgM
 +
 +
 +
 ++
 +
 +
 +
 +
 +
 +
IgA
 t
 t
 t
 t
 t
 t
 t
 t
 t
 t
IgE
 t
 t
 t
 t
 t
 Not detected

The relative Ig production was defined as followings: -, OD450 < 0.15; +, OD450 0.15–0.4; ++, OD450 0.4–0.75; +++, OD450 0.75–1.0; and ++++, OD450 > 1.0. The absorbance at 450 nm (OD450) was measured using ELISA assays. Number of animals in each treatment group = 10

Lee et al. Journal of Molecular Signaling 2008 3:12   doi:10.1186/1750-2187-3-12