Data-driven modeling of cellular stimulation, signaling and output response in RAW 264.7 cells

Yang Wu¹ , Gary L Johnson²^,3 and Shawn M Gomez¹^,4

¹Joint Department of Biomedical Engineering, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

²Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

³Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

⁴Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA

author email corresponding author email

Journal of Molecular Signaling 2008, 3:11doi:10.1186/1750-2187-3-11


Published:	22 May 2008

Additional files

Additional file 1:

Segment diagrams of the squared weighted VIP profile for G-CSF, IL-1α, IL-6, IL-10, MIP-1α and TNFα. For each cytokine, ten PLS models were generated through a 10-fold cross validation and then a weighted VIP score was computed as described in Materials and Methods to select important signaling metrics. A segment plot was produced for each protein, with the radial length of each segment indicating the magnitude of the squared weighted VIP score for individual metrics. VIP scores greater than 1 (dashed circle) are classified as significant metrics for each cytokine.

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Additional file 2:

Experimental conditions used in model creation. These conditions were identical for both protein phosphorylation state and cytokine output response measurements. Abbreviations: 2MA – 2-Methyl-thio-ATP; IFA – Interferon-alpha; IL-6 – Interleukin-6; ISO – Isoproterenol; LPA – Lysophosphatidic acid; LPS – Lipopolysaccharide; PAF – Platelet Activating Factor; PGE – Prostaglandin E2; S1P – Sphingosine-1-phosphate; UDP – Uridine 5'-diphosphate.

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