|
Resolution: standard / high Figure 1.
Dvl-3 as a marker of Wnt pathway activation. Line 30F (left panel) or co-cultures of Lines 31E + 30F (right panel) stimulated
with ectopic Wnt-3a respond with hyperphosphorylation of Dvl-3. Following treatment
with Wnt-3a for the indicated times, cells were lysed with Triton X-100 Lysis Buffer
and cell extracts were subjected to Western blot analysis. Hyperphosphorylation of
Dvl-3 is evident by a consistent relative increase in the intensity of the upper band
of the doublet. Alkaline phosphatase treatment caused the loss of the upper band (data
not shown).
Constantinou et al. Journal of Molecular Signaling 2008 3:10 doi:10.1186/1750-2187-3-10 |
