Additional File 2.Basal CLU expression in various prostate and colon carcinoma cell lines. Real time RT-PCR was used to measure basal CLU expression levels of three CLU mRNA variants in various prostate and colon carcinoma cell lines. Expression levels were normalized to the Ubiquitin C (UBC) transcript. CLU34 levels varied highly across the entire panel of cell lines, whereas CLU35 and CLU36 were consistently expressed at very low levels, in some samples below the limits of detection by the real time RT-PCR assay. Format: PDF Size: 269KB Download file This file can be viewed with: Adobe Acrobat Reader Schepeler et al. Journal of Molecular Signaling 2007 2:6 doi:10.1186/1750-2187-2-6 |