Additional File 1:

Quantitative examination of CLU-positive cells. HCT116 and LS174T cells were transfected with GFP-cyt-E-cadherin or GFP control vector. Cells were fixed and immunostained with an anti-CLU antibody 24 hr after transfection. >150 transfected (green) cells were counted and scored independently for CLU (red) up-regulation by two observers, TS and CLA.

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Additional File 2:

Basal CLU expression in various prostate and colon carcinoma cell lines. Real time RT-PCR was used to measure basal CLU expression levels of three CLU mRNA variants in various prostate and colon carcinoma cell lines. Expression levels were normalized to the Ubiquitin C (UBC) transcript. CLU34 levels varied highly across the entire panel of cell lines, whereas CLU35 and CLU36 were consistently expressed at very low levels, in some samples below the limits of detection by the real time RT-PCR assay.

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