Tyrosine-specific MAPK phosphatases expressed in PC12 cells. A) Schematic diagrams of STEP (STEP61, STEP46), HePTP and PTPRR (PTPBR7, PTP-SL, PTPPBSγ-42, PTPPBSγ-37) protein isoforms. Mouse isoform nomenclature is given on the right. Signal peptides (SP), proline-rich and PEST domains (PP), transmembrane segments (TM), kinase-interacting motifs (KIM), hydrophobic regions (HR) and catalytic phosphotyrosine phosphatase domains (PTP) are indicated. B) Assessment of KIM-containing, tyrosine-specific PTP expression by RT-PCR. Rat protein nomenclature is indicated on the left, aligned with the diagrams of the mouse orthologs depicted in panel A). STEP and HePTP transcripts, as detected by RT-PCR (+) in mouse cerebrum (CRB) and spleen (SPL) RNA, respectively, are not found in PC12 cells. For rat PTPRR proteins (PCPTP1, PCPTP1-Ce, PTPPBSγ) isoform-specific primer sets were used on rat cerebellum (CBL) and PC12 total RNA. Samples lacking RT enzyme (-) and appropriate plasmids (p) served as negative and positive controls, respectively. A β-actin specific RT-PCR served as a positive control for the reverse transcriptase reactions.
Noordman et al. Journal of Molecular Signaling 2006 1:4 doi:10.1186/1750-2187-1-4